线栓法制备大鼠局灶性脑缺血模型的失败原因探悉

目的　对大鼠局灶性脑缺血模型制备中的失败因素进行研究。方法　采用健康Wistar大鼠 ,线栓法制备MCAO(middlecerebralocclusion)模型 ,利用神经功能缺陷评分和TTC染色判定梗死是否成功。结果　夏季的大鼠梗死成活明显高于冬春季 (P <0 0 5 ) ;体重在 2 70～ 2 80g的成活率明显高于其它体重的 (P <0 0 5 ) ;雌雄之间没有明显差别 (P >0 0 5 ) ;插入长度小于 1 8cm的成活率明显高于插入长度为大于 1 8cm的 (P <0 0 5 ) ;而麻醉也影响模型成功与否 ;还未发现有明显原因而死亡的大鼠。结论　最理想大鼠脑梗死模型制备是在夏季 ,体重 2 70～ 2 80g ,插线长度小于 1 8cm。

Study of Failure Cause in Rat Focal Cerebral Ischemia Model Established by The Suture Occlusion Technique

Objective To study the failure cause during established the model of focal cerebral ischem ia in rat.Methods The healthy Wistar rat was obtained,the middle ce rebral artery occlusion (MCAO)was induced by the suture occlusion technique,to d eterminate whether the model is successful by the grade of Neurologic deficit an d the TTC stained.Results The successful rate in summer is better t han in winter and spring.(%P%<0.05).the weight 270～280 g is better than the o thers (P<0.05).the filament length <1.8 cm is better than >1.8 cm (P< 0.0 5).no significant differenceis found between the femaleand the male (P>0.05 ).Conclusion The best condition of established MCAO is rat weight 270 ～280 g,filament length <1.8 cm and in summer.