One-electron oxidation of glutathione by azide radicals in neutral medium: A gamma and pulse radiolysis study |
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| Affiliation: | 1. State Key Laboratory of Bioorganic and Natural Products Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, China;2. School of physical science and technology, ShanghaiTech University, Haike Road 100, Shanghai, 200120, China;3. University of Chinese Academy of Sciences;4. Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 26 Qiuyue Road, Shanghai 201210, China |
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| Abstract: | The selective interactions of azide radicals with glutathione (GSH) have been quantitatively studied in buffered neutral aqueous solutions using γ and pulse radiolysis. The sulfur centered GS̽ and GSSG⨪ radicals are produced in pulse and γ radiolysis. Kinetic experiments and simulation allowed to estimate the rate constant of N̽3 with GSH which has been found to be equal to (9.5 ± 0.5) × 106M−1 s−1 at pH 7. In steady state radiolysis, we have found GSSG as the final product formed with an initial G value of 2.9 × 10−7 mol J−1. |
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