Electrotransformation of Saccharomyces cerevisiae protoplast by a plasmid of Escherichia coli |
| |
| Institution: | 1. Scripps Institution of Oceanography, University of California at San Diego, La Jolla, CA, USA;2. Department of Pediatrics, University of California at San Diego, La Jolla, CA, USA;3. Collaborative to Halt Antibiotic Resistant Microbes, University of California at San Diego, La Jolla, CA, USA;4. Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, CA, USA;1. Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China;2. School of Pharmacy, Fudan University, Shanghai 201203, China;3. Department of Biochemistry, Shanghai Institute of Pharmaceutical Industry, Shanghai 201210, China;4. School of Life Science, Henan University, Kaifeng 475004, China;5. Jiangsu National Synergetic Innovation Center for Advanced Materials, SICAM, Nanjing 210009, China;6. College of Life Sciences, Shanghai Normal University, Shanghai 200232, China;1. Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, NC 27695, USA;2. Department of Food Science, University of Wisconsin-Madison, Madison, WI 53706, USA;1. Laboratory of Mycopathology and Microbial Technology, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, India;2. Department of Botany, University College of Science, Mohanlal Sukhadia University, Udaipur, Rajasthan, India |
| |
| Abstract: | The experiments reported here are aimed at obtaining optimum conditions for gene transformation after electric field pulses. Saccharomyces cerevisiae DBY746 is used as the recipient strain for shuttle plasmid (YRp group). From the relationship between the optimum electric field conditions and the transformation efficiency it is discovered that the maximum transformation efficiency appears at a wide pulse length of 400 μs with an electric field strength of 4 kV/cm, yielding up to 273 transformants/μg DNA. The electroporation unit used in the experiment is a home-made set featuring simplicity, readiness and practicality. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
