Effect of column loading on the accuracy of analyzing pentalgin N tablets by gradient high-performance liquid chromatography

Two approaches for the high-performance liquid chromatographic analysis of multicomponent medical preparations were experimentally studied by an example of Pentalgin N tablets. One of them was the simultaneous determination of all components of the preparation; another was the independent analysis of concentrated and diluted solutions for determining the presentpresent in small and large amounts, respectively. The performance characteristics of the studied procedures were determined in the analysis of model solutions containing all active and adjuvant substances of the tablets. In the simultaneous determination of all components, the relative error was smaller because of the more sustained performance of the chromatographic column in the absence of its overload with the sample observed in the independent analysis.