Determination of antibodies to Yersinia enterocolitica bacteria using a piezoelectric quartz crystal immunosensor |
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| Authors: | E. N. Kalmykova E. S. Dergunova N. Yu. Zubova R. P. Gorshkova N. A. Komandrova T. N. Ermolaeva |
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| Affiliation: | (1) Lipetsk State Technical University, ul. Moskovskaya 30, Lipetsk, 398600, Russia;(2) Center for Hygiene and Epidemiology, Lipetsk oblast, Russia;(3) Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, pr. Stoletiya Vladivostoka 159, Vladivostok-22, 69002, Russia |
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| Abstract: | A gravimetric technique is proposed for the determination of antibodies to Yersinia enterocolitica serovar O: 3 using a piezoelectric quartz crystal immunosensor based on immobilized lipopolysaccharides. Conditions are optimized and a procedure is developed for the flow-injection analysis of blood serum samples using the immunosensor as the detector. The detection limit for antibodies is found to be 1.3 μg/mL, RSD is 8%, and the analytical range is 3–100 μg/mL. The rapidity (the duration of analysis was no longer than 10 min) and the direct detection of the formed agglutinate without additional labeling are the advantages of the proposed technique. |
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