Analysis of flavonols ofSedum telephium L. leaves by capillary electrophoresis and HPLC-mass spectrometry |
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Authors: | S Sturm N Mulinacci F F Vincieri H Stuppner |
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Institution: | (1) Institute of Pharmacognosy, University of Innsbruck, Austria;(2) Department of Pharmaceutical Science, University of Florence, Italy |
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Abstract: | Summary Flavonol glycosides ofSedum telephium L. were analysed by MEKC. Baseline separation was achieved within 14 min using a fused silica capillary and a borate/phosphate
buffer solution (35 mM, pH 5.8) containing 35 mM SDS and 4% MeOH. The applied voltage was 25 kV and the thermostating temperature
was kept constant at 40°C. Injection was performed via the pressure mode for 3 s, the detection wavelength was 205 nm. The
optimized MEKC method was used for the quantitative determination of flavonol glycosides in extracts ofS. telephium leaves.
Analysis was also performed by HPLC-MS using an electrospray ionization (ESI) interface. The good agreement between the quantitative
CE results and those obtained by LC clearly demonstrated the applicability of the methods presented. |
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Keywords: | Column liquid chromatography Micellar electrokinetic chromatography Mass spectrometry Electrospray ionization Flavonol glycosides |
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