Determination of total taurine in pet foods by liquid chromatography of the dansyl derivative: collaborative study |
| |
Authors: | McCarthy K Hischenhuber C Joyce N McCarthy K Hischenhuber C Joyce N |
| |
Affiliation: | Pedigree Masterfoods, Melton Mowbray, Leicestershire, United Kingdom. |
| |
Abstract: | A liquid chromatographic (LC) method for the determination of total taurine in pet foods was evaluated in a collaborative study. Ten laboratories assayed 6 blind duplicate pairs of wet and dry pet foods. The taurine in the 6 sample pairs ranged from low (170 mg/kg) to high (2250 mg/kg) concentrations as is. Collaborators also assayed a sample of known taurine concentration for familiarization purposes. Samples were hydrolyzed to release bound taurine, which was subsequently converted to the dansyl derivative and quantitated by gradient-elution LC with fluorescence detection. Repeatability relative standard deviations, RSDr, ranged from 3.2 to 10.0%; reproducibility relative standard deviations, RSDR, ranged from 6.1 to 16.1%. The method has been adopted Official First Action status by AOAC INTERNATIONAL. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|