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En face imaging of single cell layers by differential phase-contrast optical coherence microscopy
Authors:Sticker Markus  Pircher Michael  Götzinger Erich  Sattmann Harald  Fercher Adolf F  Hitzenberger Christoph K
Abstract:Optical coherence microscopy (OCM) is capable of imaging the backscattering potential of a sample with high transversal and axial resolution. We report on a combination of OCM with a differential phase-contrast technique that permits imaging of the subwavelength optical path differences that occur between a narrow beam probing a sample and its surrounding. This technique allows small transversal refractive-index variations close to a selected interface to be seen. We report on the method and present first images of a test sample and a single cell layer. The cells act as phase objects; imaging the phase properties improves the contrast compared with that of intensity images.
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