Electrochemical transduction of an immunological reaction via s-BLMs

The application of cyclic voltammetry to biosensor devices based on solid supported bilayer lipid membranes (s-BLMs) is investigated. s-BLMs have been used for receptor-ligand contact interactions. This paper reports the electrochemical transduction of an antigen-antibody (AgAb) reaction by an s-BLM. The antigen (HBsAg) is incorporated into an s-BLM, which then can interact with its corresponding antibody (HBsAb) in the bathing solution. This AgAb interaction results in some remarkable changes in the electrical parameters (conductance, potential and capacitance) of s-BLMs. The magnitude of these changes are directly related to the concentration of the antibody in the bathing solution. The use of such an AgAb interaction via s-BLM as a transducing device for the detection of ligand-receptor contact reaction is proposed.