非标记光反射干涉生物传感器及其检测模型的构建

构建了基于反射光干涉生物传感器检测系统,用原子力学显微镜对传感单元的表面进行了表征,对检测系统传感单元进行优化,以人IgG和抗人IgG为模型建立了反射光干涉生物传感器的应用模型。结果表明:应用甩膜法构建的传感单元具有较平整的表面,以二氧化钛为干涉增强层的传感单元具有低背景信号,检测光学厚度信号与检测浓度之间具有很好的线性相关性。建立的非标记检测系统在环境、食品安全检测、药物筛选及生物材料等多方面具有广阔的应用前景。

Setup of Label-free Reflectometry Interference Spectroscopic Biosensor and It's Detecting Model

The reflectometry interference spectroscopic(RIFS)biosensor was set up.The sensing chip was characterized and optimized.The detecting model was established by the human IgG and anti-human IgG.The results show the surface of sensing chip produced by the spin-coating method is smooth.The base line signal based on the TiO2 interference enhance layer is low.There is a good linear relationship between the optical thickness and the detected concentration.This label-free detecting system has a broad application future in the environment,food safety,drug screening and biomaterials.