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Schiff Base Reaction in a Living Cell: In Situ Synthesis of a Hollow Covalent Organic Polymer To Regulate Biological Functions |
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| Authors: | Han-Bin Xu Dr. Hua-Ying Chen Dr. Jian Lv Dr. Bin-Bin Chen Ze-Rui Zhou Shuai Chang Ya-Ting Gao Wen-Fei Huang Ming-Jie Ye Zi-Jian Cheng Dr. Mahmoud Elsayed Hafez Prof. Ruo-Can Qian Prof. Da-Wei Li |
| Affiliation: | 1. Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237 P. R. China;2. Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237 P. R. China School of Science and Engineering, Shenzhen Institute of Aggregate Science and Technology, The Chinese University of Hong Kong, Shenzhen City, Guangdong, 518172 P. R. China;3. Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237 P. R. China Department of Chemistry, Faculty of Science, Beni-Suef University, Beni-Suef, 62511 Egypt |
| Abstract: | Artificially performing chemical reactions in living biosystems to attain various physiological aims remains an intriguing but very challenging task. In this study, the Schiff base reaction was conducted in cells using Sc(OTf)3 as a catalyst, enabling the in situ synthesis of a hollow covalent organic polymer (HCOP) without external stimuli. The reversible Schiff base reaction mediated intracellular Oswald ripening endows the HCOP with a spherical, hollow porous structure and a large specific surface area. The intracellularly generated HCOP reduced cellular motility by restraining actin polymerization, which consequently induced mitochondrial deactivation, apoptosis, and necroptosis. The presented intracellular synthesis system inspired by the Schiff base reaction has strong potential to regulate cell fate and biological functions, opening up a new strategic possibility for intervening in cellular behavior. |
| Keywords: | Biological Function Regulation Hollow Covalent Organic Polymers Intracellular Schiff Base Reaction in Situ Biosynthesis |