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Purification of Bioactive Lipopeptides Produced by Bacillus subtilis Strain BIA |
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| Authors: | Alajlani Muaaz Shiekh Abid Hasnain Shahida Brantner Adelheid |
| Affiliation: | 1.Department of Pharmacognosy, Institute of Pharmaceutical Sciences, University of Graz, Universitaetsplatz 4/I, 8010, Graz, Austria ;2.Department of Microbiology and Molecular Genetics, Faculty of Life Sciences, University of the Punjab, Lahore, Pakistan ;3.Department of Microbiology and Molecular Genetics, Faculty of Life Sciences, The Women University Multan, Multan, Pakistan ; |
| Abstract: | Bacillus subtilis strain BIA was used for the production of bioactive lipopeptides. Different extraction and purificationmethods were assayed as liquid–liquid extraction, and acid and ammonium sulfate precipitation followed by TLC, SPE, and gel filtration. Active fractions were further purified using RP-HPLC. The molecular mass of the purified product from HPLC was determined through Tris-Tricine SDS-PAGE and MALDI–TOF-MS. The results revealed that Bacillus subtilis strain BIA produced surfactin and iturin like compounds. Coproduction of surfactin and iturin like compounds by this strain is a remarkable trait for a potential biocontrol agent. This paper also includeds techniques that have been developed for the optimal and convenient extraction of bioactive lipopeptides from microbial origin. |
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