|
|||||
|
|
Dual-emissive Iridium(III) Complexes as Phosphorescent Probes with Orthogonal Responses to Analyte Binding and Oxygen Quenching |
|
| Authors: | Rongji Zhu Peiling Dai Jun Yang Jie Zhou Jin Zhang Prof Kenneth Yin Zhang Prof Yonghua Li Prof Shujuan Liu Prof Kenneth Kam-Wing Lo Prof Qiang Zhao |
| Institution: | 1. State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Nanjing, 210023 China
These authors contributed equally to this work.;2. State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Nanjing, 210023 China;3. Department of Chemistry, City University of Hong Kong, Hong Kong, China |
| Abstract: | Phosphorescent probes often show sensitive response toward analytes at a specific wavelength. However, oxygen quenching usually occurs at the same wavelength and thus hinders the accurate detection of analytes. In this study, we have developed dual-emissive iridium(III) complexes that exhibit phosphorescence responses to copper(II) ions at a wavelength distinct from that where oxygen quenching occurs. The complexes displayed colorimetric phosphorescence response in aqueous solutions under different copper(II) and oxygen conditions. In cellular imaging, variation in oxygen concentration over a large range from 5 % to 80 % can modulate the intensity and lifetime of green phosphorescence without affecting the response of red phosphorescence toward intracellular copper(II) ions. |
| Keywords: | Bioimaging Dual-Emission Iridium Phosphorescence Probes |