| Porous Chitosan Microcarriers for Large Scale Cultivation of Cells for Tissue Engineering： Fabrication and Evaluation |
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| 引用本文： | 陆光远,朱琳,孔丽君,张玲,公衍道,赵南明,张秀芳.Porous Chitosan Microcarriers for Large Scale Cultivation of Cells for Tissue Engineering： Fabrication and Evaluation[J].清华大学学报,2006,11(4):427-432. |
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| 作者姓名： | 陆光远 朱琳 孔丽君 张玲 公衍道 赵南明 张秀芳 |
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| 作者单位： | State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China |
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| 基金项目： | Supported by the National Key Basic Research and Development (973) Program of China (No. 2005CB623905) and the Tsinghua-Yue-Yuen Medical Science Fund |
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| 摘 要： | Introduction Regeneration of injured tissue on a man-made template has been used since the earlier 1980s1]. Afterward, it was accepted as a tissue engineering technology2,3]. Nowadays many kinds of damaged or dysfunctional tissues/organs such as bones, …
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| 关 键 词： | 多孔微载体 壳聚糖 软骨细胞 组织工程 |
| 收稿时间： | 2005-09-20 |
| 修稿时间： | 2005-09-202006-01-13 |
Porous Chitosan Microcarriers for Large Scale Cultivation of Cells for Tissue Engineering: Fabrication and Evaluation |
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| LU Guangyuan,ZHU Lin,KONG Lijun,ZHANG Ling,GONG Yandao,ZHAO Nanming,ZHANG Xiufang.Porous Chitosan Microcarriers for Large Scale Cultivation of Cells for Tissue Engineering: Fabrication and Evaluation[J].Tsinghua Science and Technology,2006,11(4):427-432. |
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| Authors: | LU Guangyuan ZHU Lin KONG Lijun ZHANG Ling GONG Yandao ZHAO Nanming ZHANG Xiufang |
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| Institution: | State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China |
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| Abstract: | Porous chitosan microspheres with diameters ranging from 180 μm to 280 μm were successfully prepared, using an anti-phase suspension method combined with temperature controlled freeze-extraction. The mean pore diameter could be regulated from 5 μm to 60 μm by varying the freezing temperature through the cooling rate. Results with in vitro chondrocyte cultures showed that cells could attach, proliferate, and spread on these porous microspheres as well as inside the microcarriers. The materials and cell co-cultures were characterized using both optical and scanning electron microscopy. These results show that the porous chitosan microspheres are promising candidates for tissue culture for use as an injectable tissue engineering scaffold. |
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| Keywords: | porous microcarrier chitosan chondrocyte tissue engineering |
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