| 毛细管电泳电致化学发光检测血浆中布比卡因 |
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| 引用本文： | 武亚艳,李甜,薛静,梁恒. 毛细管电泳电致化学发光检测血浆中布比卡因[J]. 高等学校化学学报, 2004, 25(Z1): 128-129 |
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| 作者姓名： | 武亚艳 李甜 薛静 梁恒 |
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| 作者单位： | 西安交通大学生物医学信息工程教育部重点实验室, 生命科学与技术学院分离科学研究所, 西安 710049 |
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| 基金项目： | 国家自然科学基金(批准号:20299030和20175015)资助. |
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| 摘 要： | 布比卡因是一种外科局部麻醉剂,使用过量会导致中枢神经系统和心脏血管系统中毒[1],可引起心脏停博.高效液相色谱和毛细管电泳(CE)[2]是该药常用的检测方法.
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| 关 键 词： | 布比卡因 毛细管电泳 电致化学发光 |
Capillary Electrophoresis Coupled with Tris (2,2'-bipyridyl) Ruthenium Electrochemiluminescence Detection for Bupivacaine Determination |
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| WU Ya-yan,LI Tian,XUE Jing,LIANG Heng. Capillary Electrophoresis Coupled with Tris (2,2'-bipyridyl) Ruthenium Electrochemiluminescence Detection for Bupivacaine Determination[J]. Chemical Research In Chinese Universities, 2004, 25(Z1): 128-129 |
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| Authors: | WU Ya-yan LI Tian XUE Jing LIANG Heng |
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| Affiliation: | The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Separation Science Institute, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China |
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| Abstract: | The determination of bupivacaine by capillary electrophoresis with Ru(bpy)32+ electroche-miluminescence detection was described.The detection potential,Ru(bpy)32+ concentration,phosphate buffer concentration and pH,injection voltage and time were investigated.Experiment results showed that a good separation for the bupivacaine in plasma can be achieved under the optimized conditions,such as 10 s at 10 kV of electrokinetic injection,10 mmol/L separation phosphate buffer(pH=8.0),15 kV of separation voltage,5 mmol/L Ru(bpy)32+,and 50 mmol/L phosphate buffer(pH 6.5) in the ECL cell.The linear range was 0.02-10μg/mL,the relative standard derivation(RSD) was 3.4%,and the detection limit was 3 ng/mL.When the method was applied to measure bupivacaine in rat plasma,the results were satisfactory. |
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| Keywords: | Bupivacaine Capillary electrophoresis Electrochemiluminesence |
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