氢氘交换质谱技术在蛋白质和蛋白复合物结构研究中的应用进展

蛋白质是生命功能的执行者,其功能的发挥受自身结构动态变化、与其他生物分子的相互作用及修饰等因素的调节。因此,对蛋白质及蛋白复合物结构的研究有助于揭示重要生命过程中的分子机理与机制。氢氘交换质谱(Hydrogen deuterium exchange mass spectrometry,HDX-MS)是研究蛋白质结构、动态变化和相互作用的强有力工具,也是传统生物物理手段的重要补充。该文综述了HDX-MS的基本原理、机制、实验方法和研究最新进展,并从蛋白质自身动态变化、蛋白质-小分子相互作用、蛋白质-蛋白质相互作用3个方面介绍了近年来HDX-MS在蛋白及蛋白复合物研究中的应用进展。

Application Progress of Hydrogen Deuterium Exchange Mass Spectrometry in Structural Study of Proteins and Protein Complexes

Proteins are the workhorses of cells,their biological functions are regulated by their dynamic structures,interactions with other biomolecules and many other factors.Gaining detailed structural information of proteins or protein complexes is crucial to decipher the molecular mechanisms of important biological processes.Hydrogen deuterium exchange mass spectrometry(HDX-MS) is not only a powerful tool for studying the dynamic changes of protein structures and protein ligand/protein interactions,but also an important complement to traditional biophysical approaches.In this review,the basic principles,mechanism,experimental methods and recent development of HDX-MS are presented,especially focusing on its applications in protein dynamics,protein-small molecule interactions and protein-protein interactions.