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Science based calibration for the extraction of 'analyte-specific' HPLC-DAD chromatograms in environmental analysis |
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| Authors: | Kuligowski J Galera M Martínez García M D Gil Culzoni M J Goicoechea H C Garrigues S Quintás G de la Guardia M |
| Institution: | a Department of Analytical Chemistry, Universidad de Valencia, Edificio Jerónimo Muñoz, 50th, Dr. Moliner, E-46100 Burjassot, Spain b Departamento de Hidrogeología y Química Analítica, Facultad de Ciencias Experimentales, Universidad de Almería, 04120 La Cañada de San Urbano, E-04120 Almería, Spain c Laboratorio de Desarrollo Analítico y Quimiometría (LADAQ), Cátedra de Química Analítica I, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Ciudad Universitaria, Santa Fe (S3000ZAA), Argentina |
| Abstract: | Multivariate science based calibration (SBC) has been applied to the resolution of overlapped peaks in liquid chromatography with diode array detection (LC-DAD). Complex river water samples spiked with 11 pharmaceutical substances resulted in poorly resolved chromatograms containing additional peaks from interfering matrix compounds and a change in the background absorbance due to the mobile phase gradient. Applying the present multivariate approach it was possible to resolve all 11 analytes from overlapping peaks, obtaining linear calibration lines (R2 > 0.96). Recovery percentages on spiked samples ranged between 74.6 and 113.5%, which are quite satisfactory taking into account the low concentration ranges considered to 1-7 μg L−1. |
| Keywords: | Science based calibration High performance liquid chromatography-diode array detection (HPLC-DAD) Environmental analysis |
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