Fe3+-H2O2-二氯荧光素化学发光体系测定药物中的扑热息痛

酸性介质中,Fe3+催化H2O2分解生成羟基自由基,进而氧化扑热息痛产生微弱的化学发光,二氯荧光素对该发光强度有较强的增敏作用。研究了影响化学发光强度的各种因素,并探讨了其可能的发光机理。在最佳化学发光条件下,其化学发光强度与扑热息痛的浓度在8.0×10-8～5.0×10-5mol/L范围内呈良好的线性关系,检出限为5.0×10-9mol/L,对3.5×10-6mol/L的扑热息痛平行测定9次,其相对标准偏差为2.2%。该法用于片剂中扑热息痛含量的测定,结果满意。

Determination of Paracetamol in Pharmaceuticals by Fe3+-H2O2-Dichlorofluorescein Chemiluminescence System

A novel flow-injection chemiluminescence method was developed for the determination of paracetamol,which was based on the chemiluminescence reaction between paracetamol and hydroxy free radical produced by Fe3+ and H2O2 solution in the presence of dichlorofluorescein as energy transfer in the chemiluminescence system.Various factors influencing the chemiluminescence system were investigated and the possible mechanism was also discussed.Under the optimum conditions,the linear range for the determination of paracetamol was 8.0×10-8-5.0×10-5 mol/L with a detection limit(S/N=3) of 5.0×10-9 mol/L,the relative standard deviation(RSD,n=9) was 2.2% for 3.5×10-6 mol/L paracetamol solution.The method was successfully applied in the determination of paracetamol in pharmaceutical sample.