Label-free and reagentless aptamer-based sensors for small molecules |
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Authors: | Zayats Maya Huang Ye Gill Ron Ma Chun-an Willner Itamar |
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Institution: | The Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel. |
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Abstract: | A label free, reagentless aptasensor for adenosine is developed on an ISFET device. The separation of an aptamer/nucleic acid duplex by adenosine leads to the aptamer/adenosine complex that alters the gate potential of the ISFET. The sensitivity limit of the device is 5 x 10-5 M. Also, the immobilization of the aptamer/nucleic acid duplex on an Au-electrode and the separation of the duplex by adenosine mono-phosphate (AMP) enable the electrochemical detection of adenosine by faradaic impedance spectroscopy. The separation of the aptamer/nucleic acid duplex by adenosine and the formation of the aptamer/adenosine complex results in a decrease in the interfacial electron-transfer resistance in the presence of Fe(CN)6]3-/4- as redox active substrate. |
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