pH区带精制逆流色谱法制备青风藤碱

采用pH区带精制逆流色谱法从广西地不容中制备青风藤碱，溶剂系统为石油醚 乙酸乙酯 甲醇 水（3:7:1:9,V/V），上相加10 mmol/L三乙胺为固定相，下相加10 mmol/L盐酸为流动相。一次进样1.0 g，可得纯度为99.5%的青风藤碱600 mg，所得产物经ESI-MS，¹H NMR和¹³C NMR进行结构鉴定。本方法效率高，制品纯度高，适合青风藤碱的大量制备。

Preparation of sinoacutine from Stephania kwansiensis by pH-zone-refining counter-current chromatography

We employed pH-zone refining counter-current chromatography to prepare high-purity sinoacutine from Stephania kwangsiensis. Its solvent system was composed of petroleum ether-ethyl acetate-methanol-water （3 ： 7： 1 ： 9, V/V）, in which triethylamine （ 10 mmol/L） was added to the upper organic stationary phase as a retainer and hydrochloric acid （10 mmol/L） was added to the aqueous mobile phase as an eluter. The structure of sinoautine was identified by ESI-MS,1H NMR and 13C NMR. The purity of sinoacutine determined by RP-HPLC was 99.5%. This approach has such advantages as high-efficiency and high-purity, suitable for the preparation of large amount of sinoacutine.