Oriented immobilization of a fully active monolayer of histidine-tagged recombinant laccase on modified gold electrodes |
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| Authors: | Balland Véronique Hureau Christelle Cusano Angela Maria Liu Yingli Tron Thierry Limoges Benoît |
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| Institution: | Laboratoire d'Electrochimie Moléculaire, UMR CNRS 7591, Université Paris Diderot, 2 place Jussieu, Paris Cedex 05, France. veronique.balland@univ-paris-diderot.fr |
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| Abstract: | The formation of a dense monolayer of histidine-tagged recombinant laccase on gold electrodes by using a short thiol-NTA linker is described, as well as a kinetic analysis of the process by cyclic voltammetry. From a detailed analysis of the catalytic reduction of dioxygen by laccase in the presence of a one-electron redox mediator it can be concluded that the immobilized enzyme remains as active as in homogeneous solution. |
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| Keywords: | electrochemistry enzymes immobilization redox mediators self‐assembled monolayers |
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