| MALDI-TOF质谱、电泳和透射电子显微镜技术研究魟鱼肝铁蛋白H和L亚基特性 |
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| 引用本文： | 黄河清,胡晓慧,方雪萍,曹廷明,孔波.MALDI-TOF质谱、电泳和透射电子显微镜技术研究魟鱼肝铁蛋白H和L亚基特性[J].分析化学,2009,37(5). |
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| 作者姓名： | 黄河清 胡晓慧 方雪萍 曹廷明 孔波 |
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| 作者单位： | 1. 厦门大学生命科学学院生物化学与生物技术学系,厦门,361005;海洋与环境科学学院近海海洋科学国家重点实验室,厦门,361005;化学化工学院化学生物学系,厦门,361005
2. 厦门大学生命科学学院生物化学与生物技术学系,厦门,361005 |
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| 摘 要： | 小批量制备质谱纯魟鱼肝铁蛋白(liver ferritin of Dasyatis akajei, DALF),以透射电子显微镜术(TEM)测定DALF、蛋白壳和铁核的分子尺寸.SDS-PAGE技术指出,DALF由H 和L两种不同类型的亚基组成.采用肽质量指纹图谱(PMF)技术进一步佐证H和L亚基类型和同源性.氧化还原剂中性红、劳氏紫、甲基紫精和 pH 1.5酸度均无法削弱DALF的H-L和L-L亚基之间的相互作用强度、并解吸出L亚基离子,供MALDI-TOF质谱仪分析.通过增加激光强度和降低基质pH途径,可直接分析DALF的H和L亚基的分子量,指出DALF中H-L和L-L亚基之间的相互作用强度明显高于H-H亚基类型.
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| 关 键 词： | 铁蛋白 亚基类型 电镜 质谱 电泳 |
Characteristics of H and L Subunits with Mass Spectrometry,Electrophoresis and Transmission Electron Microscopy in Liver Ferritin of Dasyatis Akajei |
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| HUANG He-Qing,HU Xiao-Hui,FANG Xue-Ping,CAO Ting-Ming,KONG Bo.Characteristics of H and L Subunits with Mass Spectrometry,Electrophoresis and Transmission Electron Microscopy in Liver Ferritin of Dasyatis Akajei[J].Chinese Journal of Analytical Chemistry,2009,37(5). |
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| Authors: | HUANG He-Qing HU Xiao-Hui FANG Xue-Ping CAO Ting-Ming KONG Bo |
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| Institution: | Department of Biochemistry and Biotechnology;School of Life Sciences1;State Key Laboratory of Marine EnvironmentalScience;College of Oceanography and Environmental Science2;The Key Lab.of Chemical Biology of Fujian Province;College of Chemistry & Chemical Engineering3;Xiamen University;Xiamen 361005 |
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| Abstract: | Liver ferritin of Dasyatis akajei(DALF) with mass purity was prepared in batch.Transmission electron microscopy(TEM) was used to determine the molecular sizes of DALF,protein shell,and iron core,respectively.Experimental results of SDS-PAGE method indicated that DALF consisted of two different subunit types,H and L subunits.Moreover,both types of H and L subunits and their homology were further proofed by peptide mass fingerprinting(PMF).The redox reagents such as natural read,thionine,and methyl viologen,a... |
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| Keywords: | Ferritin subunit type electron microscopy mass spectrum electrophoresis |
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