A fluorescent probe for detecting thiamine using the luminescence intensity of nanoparticles

Determination of molecules and biomolecules using nanoparticles is promising in the development of analytical techniques. Modified Eu-doped Y₂O₃ nanoparticles (Y₂O₃:Eu NPs) by captopril have been used as a probe for thiamine (vitamin B₁) determination. According to the fluorescence enhancement of modified Eu-doped Y₂O₃ nanoparticles caused by thiamine, a simple and sensitive method were proposed for its detection. The increase in modified Y₂O₃:Eu NPs fluorescence signal as a function of thiamine concentration was found to be linear in the concentration range of 0–44 μM. The limit of detection (LOD) of thiamine by this method was 0.144 μM. All the measurements were performed in natural pH, at the room temperature under ambient conditions. Possible interaction mechanism was discussed.