Evaluation of different carbon and nitrogen sources in production of rhamnolipids by a strain of Pseudomonas aeruginosa |
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Authors: | Santos Alexandre S Sampaio Ana Paula W Vasquez Gina S Santa Anna Lídia M Pereira Nei Freire Denise M G |
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Institution: | (1) Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Lab 549-2, CEP: 21945-900 Rio de Janeiro, Brazil;(2) Brazilian Oil Research Center (Petrobras), CENPES, Brazil;(3) Department of Biochemical Engineering, School of Chemistry, UFRJ, Brazil;(4) Faculty of Pharmacy, Federal University of Rio de Janeiro, Brazil |
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Abstract: | Culture conditions involving variations in carbon and nitrogen sources and different C:N ratios were examined with the aim
of increasing productivity in the process of rhamnolipid synthesis by Pseudomonas aeruginosa. In addition to the differences in productivity, the use of different carbon sources resulted in several proportions related
to the types of rhamnolipids synthesized (monorhamnolipids and dirhamnolipids). Furthermore, the variation in nutrients, mainly
the nitrogen source, resulted in different amounts of virulence factors, as phenazines and extracellular proteins. The data
point out a new concern in the choice of substrate to be used for rhamnolipid production by P. aeruginosa: toxic byproducts. |
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Keywords: | Rhamnolipids biosurfactant virulence factors Pseudomonas aeruginosa |
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