Resolution of nucleic acids by high-performance liquid chromatography |
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| Affiliation: | 1. Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China;2. Department of Pharmaceutical and Biomedical Sciences, The University of Georgia College of Pharmacy, 250 W. Green Street, Athens, GA, 30602-2352, USA;3. Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Ain Shams University, Organization of African Unity Street, Abassia, Cairo, 11566, Egypt;4. Micromeritics Instrument Company, 4356 Communications Drive, Norcross, GA, 30093, USA;5. ProQR Therapeutics, Zernikedreef 9, 2333 CK, Leiden, Netherlands |
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| Abstract: | High-performance liquid chromatography is a very powerful technique for the separation and isolation of nucleic acids. Nucleic acids can be generally characterized as long, negatively charged polymers with hydrophobic nucleobases. Chromatographic processes which use electrostatic interactions (anion-exchange), hydrophobic interactions (reversed-phase or salting-out) or both types of interactions (mixed-mode) are most effective for resolution of these materials. |
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