Application of reversed-phase high-performance liquid chromatography for structural characterization of abnormal hemoglobins |
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| Institution: | 1. Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570006 Karnataka, India;2. Centre for Rural Development and Technology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016 India;1. Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, PO 11562 Cairo, Egypt;2. Analytical Chemistry Department, Faculty of Pharmacy, Modern Technology and Information University, Cairo, Egypt;3. Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy and Drug Technology, Egyptian Chinese University, Cairo, Egypt;4. Basic and Applied Sciences Institute, Egypt-Japan University of Science and Technology (E-JUST), New Borg El-Arab City, PO 21934 Alexandria, Egypt;5. Analytical Chemistry Department, Faculty of Pharmacy, October 6 University, 6 October City, Egypt |
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| Abstract: | The various steps for the structural analysis of a hemoglobin molecule (protein isolation, peptide purification, amino acid composition) can be achieved by the unique use of high-performance liquid chromatography. Reversal-phase high-performance liquid chromatography is of special interest for the identification of neutral variants of hemoglobin. |
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