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| 超顺磁性免疫磁珠体系用于植物油中黄曲霉毒素B1的检测研究 | |
| 引用本文： | 刘伟伟,孙秀兰,张银志,樊惠良,陈文君,李在均. 超顺磁性免疫磁珠体系用于植物油中黄曲霉毒素B1的检测研究[J]. 分析测试学报, 2011, 30(12): 1345-1350. DOI: 10.3969/j.issn.1004-4957.2011.12.003 |
| 作者姓名： | 刘伟伟 孙秀兰 张银志 樊惠良 陈文君 李在均 |
| 作者单位： | 1. 江南大学食品科学与技术国家重点实验室,江苏无锡214122;江南大学食品学院,江苏无锡214122 2. 江南大学食品学院,江苏无锡,214122 3. 张家港出入境检验检疫局,江苏张家港,215500 4. 江南大学化学与材料工程学院,江苏无锡,214122 |
| 基金项目： | 国家自然科学基金资助项目（20806033）；张家港市科技局资助项目（ZKS0904） |
| 摘 要： | 利用化学共沉淀方法,制得了主要成分为Fe3O4的超顺磁性纳米磁珠。纳米磁珠和硅烷偶联剂氨丙基三乙氧基硅烷(APTES)反应而带上氨基基团。带上氨基的磁珠通过戊二醛活化后发生醛基化,与黄曲霉毒素B1(AFB1)多克隆抗体偶联得到黄曲霉毒素B1免疫磁珠。利用该免疫磁珠为净化工具,建立了有机溶剂萃取、免疫磁珠净化、高效液相色谱(HPLC)检测植物油中黄曲霉毒素B1的方法。该方法具有良好线性,线性范围为5～50μg/L,相关系数达0.999 4,检出限为0.5μg/L,平均回收率为96%,相对标准偏差为12.5%。该方法操作简单、灵敏度高、准确性好,可用于植物油中黄曲霉毒素B1的检测。 |
| 关 键 词： | 免疫磁珠 植物油 黄曲霉毒素B1 |
Research of the Super Paramagnetic Immune Magnetic Beads System for Detection of Aflatoxin B1 in Vegetable Oil |
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| LIU Wei-wei,SUN Xiu-lan,ZHANG Yin-zhi,FAN Hui-liang,CHEN Wen-jun,LI Zai-jun. Research of the Super Paramagnetic Immune Magnetic Beads System for Detection of Aflatoxin B1 in Vegetable Oil[J]. Journal of Instrumental Analysis, 2011, 30(12): 1345-1350. DOI: 10.3969/j.issn.1004-4957.2011.12.003 | |
| Authors: | LIU Wei-wei SUN Xiu-lan ZHANG Yin-zhi FAN Hui-liang CHEN Wen-jun LI Zai-jun |
| Affiliation: | LIU Wei-wei1,2,SUN Xiu-lan1,ZHANG Yin-zhi2,FAN Hui-liang3,CHEN Wen-jun3,LI Zai-jun4(1.State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China,2.School of Food Science and Technology,3.Entry-Exit Inspection and Quarantine Bureau,Zhangjiagang 215500,4.School of Chemical and Material Engineering,China) |
| Abstract: | Using the method of chemical co-precipitation,the super paramagnetic nanoparticles with the key component of Fe3O4 were synthesized.The magnetic beads reacted with the silane coupling agent 3-aminopropyl triethoxysilane and connected with amino groups.After activated by the agent glutaraldehyde,the magnetic beads took along the aldehyde group and coupled with aflatoxin B1 polyclonal antibody to form the immune magnetic beads.A method was established to detect the aflatoxin B1 in vegetable oil by using the o... |
| Keywords: | immune magnetic beads vegetable oil aflatoxin B1 |
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