A dual enzyme-phosphate hybrid nanoflower for glutamate detection |
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| Affiliation: | 1. State Key Laboratory of Food Nutrition and Safety, Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China;2. Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhang, 050000, China;3. Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60695, Poznan, Poland;1. Chemical Engineering & Process Development Division, CSIR-National Chemical Laboratory, Pune, 411008, India;2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India;3. University College London, Torrington Place, London, WC1E 7JE, UK;1. School of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China;2. Center for Particle-Laden Turbulence, Key Laboratory of Mechanics on Disaster and Environment in Western China attached to the Ministry of Education of China, Department of Mechanics and Engineering Science, School of Civil Engineering and Mechanics, Lanzhou University, Lanzhou, 730000, China;1. College of Chemistry and Chemical Engineering, Guizhou University, Guiyang, 550025, China;2. Collaborative Innovation Center of Guizhou Province for Efficient Utilization of Phosphorus and Fluorine Resources, Guizhou University, Guiyang, 550025, China;1. Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming, 650093, China;2. State Key Laboratory of Complex Nonferrous Metal Resources Cleaning Utilization in Yunnan Province, Kunming, 650093, China |
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| Abstract: | The enzyme hybrid nanoflower has gained interests in biosensors due to their simple synthesis and high efficiency. In this study, glutamate oxidase (GLOX) and horseradish peroxidase (HRP) hybrid nanoflowers (GLOX&HRP-HNFs) were successfully prepared for the detection of glutamic acid (Glu). The effects of the synthesis conditions on the activity of GLOX & HRP-HNFs were investigated. Results revealed that the maximum activity of GLOX&HRP-HNFs was under 4 mM phosphate radical, 2.5 mM MnSO4, 0.04 mg/mL GLOX, and 0.16 mg/mL HRP. After immobilization, no significant differences were observed in optimum pH and temperature values of the GLOX and HRP. The GLOX&HRP-HNFs exhibited higher storage stability and resistance to organic solvents than free GLOX and HRP. Additionally, the GLOX&HRP-HNFs maintained 69% of its primary activity after 6 cycles. More important, the GLOX&HRP-HNFs exhibited a good linear range from 1 to 100 μM (R2 = 0.9979) and a low limit of detection (LOD) of 0.59 μM for glutamate. These results suggest that the GLOX&HRP-HNFs is a promising candidate for applications in biosensing for the detection of glutamate. |
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| Keywords: | Glutamate oxidase Horseradish peroxidase Nanoflowers Glutamate detection |
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