外源核苷酸对大菱鲆幼鱼生长和溶菌酶活力的影响

【目的】研究外源核苷酸对大菱鲆(Scophthalmus maximus)幼鱼生长和免疫力的影响,为核苷酸营养在大菱鲆养殖上的推广应用提供依据。【方法】以鱼粉为主要蛋白源,在基础饲料中分别添加不同含量的核苷酸,添加量(W/W)分别为0.00%,0.01%,0.03%,0.10%,0.30%,0.50%。在人工养殖的情况下(自然光照,水温11.0~16.3℃),对初始体质量为12.07~12.29g的大菱鲆幼鱼进行核苷酸营养的外源添加实验,分别在4周和8周对不同组别的生长和生态转化效率进行定期测定。然后在8周实验结束时,对大菱鲆血清中溶菌酶活力进行测定。【结果】随着在基础饲料中核苷酸添加量的增加,大菱鲆的特定生长率逐渐减小,且大菱鲆生态转化效率实验组小于对照组,而血清中溶菌酶活性实验组高于对照组。【结论】核苷酸对大菱鲆幼鱼的生长无明显的促进或抑制作用,对血清中的溶菌酶活性具有促进作用。

Effect of a Nucleotide-enriched Diet on the Growth and Lysozyme Activity in Juvenile Turbot (Scophthalmus maximus)

Objective]The research of exogenous nucleotides (EN)effecting on growth and in-nate immunity of j uvenile turbort (Scophthalmus maximus),which provides the evidence for the popularization and application of Scophthalmus cultivation.Methods]With a basal di-et formulated from fish meal containing 47% crude protein and 1 1% lipid by adding exoge-nous nucleotides of 0.00%,0.01%,0.03%, 0·10%,0·30%,0.50%,respectively (W/W ). Each diet was distributed by hand to triplicate groups of 1 5 fish and the growth trial lasted 8 weeks.Results]At the end of 4 weeks and 8 weeks,the special growth rate and ecological conversion efficiencies were the same for all groups. Although a reverse relationship was found between the special growth rate and EN levels,also,a weaker relationship was found between the ecological conversion efficiencies and EN levels.The activity of lysosomal enzymes is promoting.Conclusion]We conclude that,EN is not having positive or negative on growth rate of S.maximus,at least at the stage of juvenile.However,elevated activity of lysosomal enzymes in blood serum indicates its promotion of innate immunity.