Nonlinear Excitation of Tryptophan Emission Enhanced by Silver Nanoparticles |
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| Authors: | Diego Rativa Anderson S L Gomes Sebastian Wachsmann-Hogiu Daniel L Farkas Renato E de Araujo |
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| Institution: | (1) Department of Electronic and Systems, Universidade Federal de Pernambuco, Recife, PE, Brazil;(2) Department of Physics, Universidade Federal de Pernambuco, Recife, PE, Brazil;(3) Minimally Invasive Surgical Technologies Institute and Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA, USA;(4) NSF Center for Biophotonics Science and Technology, University of California Davis, Sacramento, CA, USA |
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| Abstract: | We measured and analyzed the behavior of the fluorescence of tryptophan water solutions with and without silver nanoparticles,
excited by one, two and three photon processes. Two different colloids with silver nanoparticles with distinct diameters (0.65 nm
and 9 nm) were used in the experiments. Fluorescence quenching was observed with one and two photon excitation. However, upon
three-photon excitation, significant fluorescence enhancement was observed in the colloid. In this case excitation of the
amino acid is assisted by the nonlinear absorption of infrared light by the silver nanoparticles. In this paper we are proposing
a new way to explore metallic nanoparticles to enhance autofluorescence of biomolecules. |
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| Keywords: | Tryptophan Autofluorescence Metallic nanoparticles Nonlinear excitation |
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