Direct Electrochemistry of Glucose Oxidase Immobilized at a Novel Composite Material β‐Cyclodextrin/poly(4‐aminothiophenol)/Au Nanoparticle Modified Electrode |
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Authors: | Qing‐Lin Sheng Kai Luo Rui‐Xiao Liu Jian‐Bin Zheng |
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Institution: | Institute of Analytical Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi'an, Shaanxi, 710069, P. R. China |
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Abstract: | A novel complex material was fabricated by three steps. In the first step, gold nanoparticle (Aunano) was prepared with the method of chemistry and dialysis. In the second step, 4‐aminothiophenol (AT) was encapsulated in the cavity of β‐cyclodextrin and formed inclusion complex, cyclodextrin/4‐aminothiophenol (CD/AT). And then inclusion complex was adsorbed to the surface of Aunano based on the bond of Au‐S interaction. In the last step, a complex material, cyclodextrin/poly(4‐aminothiophenol)‐Au nanoparticles (CD/PAT‐Aunano) was obtained by the polymerizing in the acid solution initiated by chlorauric acid. The CD/PAT‐Aunano has spherical nanostructure with the average diameter of 55 nm. Glucose oxidase (GOx) was anchored with this complex material and direct electrochemistry of GOx was achieved. A couple of stable and well‐defined redox peaks were observed with the formal potential (E0′) of ‐0.488 V (vs. SCE) in a pH 6.98 buffer solution. The GOx modified electrode also exhibited an excellent electrocatalytic activity to the reduction of glucose, a linearity range for determination of glucose is from 0.25 mM to 16.0 mM with a detection limit of 0.09 mM (S/N = 3). This protocol had potential application to fabricate the third‐generation biosensor. |
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Keywords: | Biosensor Direct electrochemistry Glucose oxidase Glucose Functionalized Au nanoparticles |
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