Ex-vivo NMR of unprocessed tissue in water: a simplified procedure for studying intracranial neoplasms |
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| Authors: | Maosheng Xu Jieru Ye Daiwen Yang Xiaoli Xu Tseng Tsai Yeo Wai Hoe Ng C C Lim |
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| Institution: | (1) Department of Neuroradiology, National Neuroscience Institute, 11 Jalan Tan Tock Seng, Singapore, 308433, Singapore;(2) Department of Radiology, The Affiliated First Hospital of Zhejiang Chinese Medical University, Zhejiang, China;(3) Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore, 117543, Singapore;(4) Nuclear Magnetic Resonance (NMR) Facility, Institute of Molecular and Cell Biology, 61 Biopolis Drive (Proteos), Singapore, 138673, Singapore;(5) Department of Neurosurgery, National Neuroscience Institute, 11 Jalan Tan Tock Seng, Singapore, 308433, Singapore |
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| Abstract: | Ex-vivo and in-vitro nuclear magnetic resonance (NMR) spectroscopy techniques have been used for studying chemical metabolites
in surgically resected specimens of human neoplasms, and may provide complementary information to in-vivo whole-body magnetic-resonance
spectroscopy (MRS). We describe an ex-vivo NMR in water method for measurement of water-soluble metabolites in unprocessed
normal rat brain tissue and human intracranial neoplasms. The NMR spectra obtained using the method described here were comparable
to those obtained using high-resolution magic-angle spinning (HRMAS) NMR methods, with good correlation in metabolite concentrations
relative to creatine (r
2 = 0.7635). Improved spectral resolution and baseline were noted compared to HRMAS, but macromolecule resonances were not
detected. Ex-vivo NMR of unprocessed tissue in water is rapid and technically simple to perform, and has the potential to
be used for direct assessment of intracranial neoplasms. |
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| Keywords: | Nuclear magnetic resonance Brain neoplasm Rat brain High-resolution magic-angle spinning Choline |
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