| 高效液相色谱法测定盐酸芬氟拉明及其在片剂中含量 |
| |
| 引用本文： | 钱小平,赵远征,孙丽媛.高效液相色谱法测定盐酸芬氟拉明及其在片剂中含量[J].色谱,1998,16(6):548-549. |
| |
| 作者姓名： | 钱小平 赵远征 孙丽媛 |
| |
| 作者单位： | 大连市药品检验所，大连制药厂 |
| |
| 摘 要： | 应用高效液相色谱法测定了盐酸芬氟拉明及其片剂的含量，使用２５ｃｍ×０．４６ｃｍｉ．ｄ．分析柱，填充ＨｙｐｅｒｓｉｌＢＤＳＣ１８（５μｍ），检测波长２６４ｎｍ，流动相为Ｖ（乙腈）∶Ｖ（０．４ｍｏｌ／Ｌ乙酸铵）∶Ｖ（三乙胺）＝３０∶７０∶２。选用咖啡因作内标物，用内标法进行定量测定，在０．１～０．５ｇ／Ｌ范围内呈良好的线性关系，相关系数为０．９９９９。片剂的平均回收率为９９．９８％。方法简单、快速、灵敏度高、重现性好。
|
| 关 键 词： | 高效液相色谱法 咖啡因 盐酸芬氟拉明 |
Quantitative Determination of Fenfluramine Hydrochloride and Its Tablets by High Performance Liquid Chromatography |
| |
| Qian Xiaoping,Zhao Yuanzheng and Sun Liyuan.Quantitative Determination of Fenfluramine Hydrochloride and Its Tablets by High Performance Liquid Chromatography[J].Chinese Journal of Chromatography,1998,16(6):548-549. |
| |
| Authors: | Qian Xiaoping Zhao Yuanzheng and Sun Liyuan |
| |
| Institution: | Dalian Institute for Drug Control, Dalian, 116021. |
| |
| Abstract: | Fenfluramine hydrochloride and its tablets were determined with high performance liquid chromatography by using an analytical column (25 cm x 0.46 cm i.d.) packed with Hypersil BDS C18 (5 microns), a UV detector at 264 nm and V(acetonitrile): V(0.4 mol/L ammonium acetate): V(triethylamine) = 30:70:2 as eluent. The quantitative determination was performed with caffeine as the internal standard. The internal standard method showed good linearity in the mass concentration range of 0.1-0.5 g/L with r = 0.9999. The average recovery of fenfluramine tablets was 99.98%. This method is simple, rapid, sensitive and reproducible. |
| |
| Keywords: | NULL |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《色谱》浏览原始摘要信息 |
| 点击此处可从《色谱》下载免费的PDF全文 |
