| 高效液相色谱法测定阿弗菌素含量 |
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| 引用本文： | 邵斌贝,胡凤祖,师治贤.高效液相色谱法测定阿弗菌素含量[J].色谱,1998,16(1):87-88. |
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| 作者姓名： | 邵斌贝 胡凤祖 师治贤 |
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| 作者单位： | 中国科学院西北高原生物研究所 |
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| 摘 要： | 采用反相高效液相色谱法测定了阿弗菌素片中阿弗菌素的含量。色谱柱为ＴＳＫ－ＧＥＬＣ１８，流动相为甲醇∶水（７５∶２５），检测波长２４５ｎｍ，可得到满意的分离效果。
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| 关 键 词： | 阿弗菌素 高效液相色谱法 |
Determination of Avermictin in Avermictin Tablet by High Performance Liquid Chromatography |
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| Shao Yun,Hu Fengzu and Shi Zhixian.Determination of Avermictin in Avermictin Tablet by High Performance Liquid Chromatography[J].Chinese Journal of Chromatography,1998,16(1):87-88. |
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| Authors: | Shao Yun Hu Fengzu and Shi Zhixian |
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| Institution: | Northwest Plateau Institute of Biology, the Chinese Academy of Sciences, Xining, 810001. |
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| Abstract: | A method for the separation and determination of avermictin in avermictin tablet by reversed-phase high performance liquid chromatography (RP-HPLC) was developed. Operating conditions were as follows: TSK-GEL C18 column, 250 mm x 4.6 mm i.d., MeOH-H2O (75:25, V/V) mobile phase with a flow rate of 1.0 mL/min, and UV detection at 245 nm. The results showed that the active constituents avermictin B1a and B1b can by separated effectively. The relative standard deviations were 2.56% and 1.31%, respectively (n = 6). The method is accurate, rapid and simple, and provides a scientific basis for industrial production and quality control of avermictin tablet. |
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| Keywords: | high performance liquid chromatography avermictin |
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