N-乙酰-1,6-二氨基己烷的固定化及其诱导肿瘤细胞分化机理的研究

合成了Ｎ－乙酰－１，６－二氨基己烷（Ｎ－ａｃｅｔｙｌ－１，６－ｄｉａｍｉｎｏｈｅｘａｎｅ，ＮＡＤＡＨ）并偶联到甘油氧丙基硅胶上用以制备高效液相色谱柱，用液相色谱法研究了ＮＡＤＡＨ及六亚甲基二乙酰胺（ｈｅｘａｍｅｔｈｙｌｅｎｅｂｉｓａｃｅｔａｍｉｅｄ，ＨＭＢＡ）诱导肿瘤细胞分化的机理。结果表明ＮＡＤＡＨ对肿瘤细胞的成分无离子交换及亲和效应，而对一些膜蛋白提取物和ＤＮＡ有强的疏水作用。因此，ＮＡＤＡＨ和ＨＭＢＡ对肿瘤细胞的诱导分化与它们对蛋白质和ＤＮＡ的疏水作用有关。

Immobilization of N Acetyl 1,6 Diaminohexane(NADAH) on Diol Silica and Study of Mechanism of NADAH and Hexamethylene Bisacetamide(HMBA)Inducing Tumor Cells Differentiation

A method for separating NADAH and its immobilization onto diol-silica have been developed. HMBA and its meabolite NADAH were used as the inducers for many types of tumor cells' differentiation. The inducing mechanism of HMBA is not clear yet. Experiments show that HMBA and NADAH have a relatively strong hydrophobic reaction and hardly ion-exchange with some DNA and some proteins of the cytosolic fraction of HL-60 human acute progranulocytic leukemia cells, BIU-87 human bladder carcinoma cells and the human erythrocyte membrane, when utilizing immobilization of NADAH. The retention time of the proteins and DNA was longer than that of the phosphatides. These results show that the adsorption of HMBA and NADAH with some proteins and DNA is higher than that with the phosphatides. The expected receptor binding biospecifically with HMBA has not been found.