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疏水色谱法的进展及其在生化研究中的应用
引用本文:刘彤,耿信笃.疏水色谱法的进展及其在生化研究中的应用[J].色谱,1998,16(1):30-34.
作者姓名:刘彤  耿信笃
作者单位:西北大学现代分离科学研究所
摘    要:着重评述了疏水色谱法的理论研究及疏水固定相中无机填料、有机填料、非多孔填料以及大孔膜的新发展,并对疏水色谱法在生物大分子的分离、纯化和生化研究中的应用,包括在蛋白质复性、折叠和分子构象变化等方面的应用作了介绍,全文包括62篇文献和一张表格。

关 键 词:分离与纯化  固定相  计量置换保留模型  生物大分子  疏水相互作用色谱法  

New Development of Hydrophobic Interaction Chromatography and Its Applications in Biochemical Research
Liu Tong and Geng Xindu.New Development of Hydrophobic Interaction Chromatography and Its Applications in Biochemical Research[J].Chinese Journal of Chromatography,1998,16(1):30-34.
Authors:Liu Tong and Geng Xindu
Institution:Institute of Modern Separation Science, Northwest University, Xi'an, 710069.
Abstract:This review is mainly concerned with the recent developments in (i) the retention mechanism of biopolymers in hydrophobic interaction chromatography (HIC); (ii) the synthesis of HIC packings, such as, the packings based on the inorganic and organic matrices, non porous packings and porous membranes; (iii) the applications of HIC in both purifications of biopolymers and biochemical research, such as, renaturation and refolding of some denatured proteins, the change in molecular conformation of biopolymers. The review includes 62 references and a table.
Keywords:hydrophobic interaction chromatography  stoichiometric displacement model  packings  biopolymer  separation and purification
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