高效液相色谱法分析矿泉水中酚类化合物

用带荧光检测器的高效液相色谱法同时测定了矿泉水中的苯酚、甲酚、双酚Ａ、双酚Ｆ、４－叔丁酚、双酚Ａ二环氧甘油醚和双酚Ｆ二环氧甘油醚等，水样经液－固萃取浓缩后再进行分析，检测限可达０．１～０．２μｇ／Ｌ。重复测定的标准偏差为１．１２％～１３．２１％，水样测定的回收率为８１％～１０５％。

Analysis of Phenolic Compounds in Mineral Water by High Performance Liquid Chromatography(HPLC)

A high performance liquid chromatographic method for the simultaneous analysis of phenolic compounds, such as, phenol, m-cresol, bisphenol A, bisphenol F, 4-tert-butylphenol, bisphenol F diglycidyl ether (BFDGE) and bisphenol A diglycidyl ether (BADGE) in mineral water with fluorometric detector is described in this paper. After pretreatment through solid-liquid extraction, the sample was analyzed by HPLC. The operating conditions were Turner C18, 5 microns, column, 150 mm x 4.6 mm i.d., mobile phase with V(ACN): V(water) = 40:60 for 10 min, then linear gradient elution for 25 min up to 80:20 with a flow rate of 1.3 mL/min. Excitation wavelength was set at 275 nm and emmission at 300 nm. The recoveries were 81%-105% and the RSDs were 1.12%-13.21%. Detection limit reached 0.1-0.2 microgram/L.