反相高效液相色谱法测定补钙保健品中的维生素D_3

 用氯仿作提取剂直接提取补钙保健品中的维生素Ｄ３,提取液经浓缩后进行高效液相色谱分析,并对皂化和直接提取的样品中维生素Ｄ３的含量进行了比较。采用μ－ＢｏｎｄａｐａｋＣ１８作分离柱,甲醇－水（８７１３）作流动相,检测波长为２６４ｎｍ,回收率为９５．６３％。

Determination of vitamin D3 in health-care foods fortified with calcium by reversed-phase high performance liquid chromatography]

"Bu Gai Bao" and "Gaoxiao Man Gai" are health-care foods fortified with calcium for people of all ages. This paper describes a method of reversed-phase high performance liquid chromatography for quantitating vitamin D3 in these products. The method is simple, sensitive, and suitable for routine analysis of vitamin D3 in a variety of health-care foods fortified with calcium. Shimadzu LC-4A high performance liquid chromatograph with SPD-2AS UV detector and C-R2AX data processor were used. The vitamin D3 in products was extracted with chloroform and separated with mu-Bondapak C18 column(300 mm x 3.9 mm i.d.) and methanol water(87:13, V/V) mobile phase with detection at UV-264 nm. Regression coefficient of correlation of calibration curves in the range of 10-50 mg/L was 0.9998. Average recoveries of vitamin D3 added to the products was 95.63%. The coefficients of variation of vitamin D3 in both products were 0.24%-0.49%.