毛细管气相色谱法对大麻中主要成分的定性定量分析

采用毛细管气相色谱法测定大麻中大麻酚、四氢大麻酚和大麻二酚的含量。以氯仿为提取溶剂，甲醇为色谱溶剂，用ＨＰ－５（１０ｍ×０．５３ｍｍ×２．６５μｍ）柱，以柱温２２０℃进行测定。大麻二酚、四氢大麻酚和大麻酚在２０～１２０ｍｇ／Ｌ的浓度范围内线性关系良好，ｒ分别为０．９９９４，０．９９９１和０．９９９５，回收率分别为９７．３％～１０４．０％，９７．３％～１０６．６％和９５．３％～１０２．４％，最低检测限均为０．２μｇ／ｍＬ。利用３种主要成分保留时间的良好重现性也可进行定性。方法简便、快速、准确、灵敏。

Analysis of the Major Components in Cannabis by Capillary Gas Chromatography

A capillary GC method for the simultaneous determination of cannabidiol (CBD), tetrahydrocannabinol (THC), and cannabinol (CBN) in cannabis is described. The major components in cannabis were extracted with chloroform. After being concentrated by evaporation, the residue was dissolved in methanol. The resulting solution was used for analysis. The conditions for determination were: FID detector, HP-5 capillary column, 10 m x 0.53 mm x 2.65 microns film, column temperature 220 degrees C. The recoveries of the three major components were 97.3%-104.0%(CBD), 97.3%-106.6%(THC) and 95.3%-102.4% (CBN). The coefficients of variation are less than 3%. The limit of detection for the three components was 0.2 mg/L, and their linearity was in the range of 20-120 mg/L. Identification of each component was achieved by comparison of retention times with the standards. This method has the advantages of simplicity, rapidity, sensitivity and accuracy.