Evaluation of important fatty acid ratios in poultry feed lipids by ATR FTIR spectroscopy |
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| Authors: | S.A. Mahesar S.T.H. Sherazi A.A. Kandhro M.I. Bhanger A.R. Khaskheli M.Y. Talpur |
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| Affiliation: | aNational Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan;bPakistan Council of Scientific & Industrial Research Laboratories, Karachi 75280, Pakistan |
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| Abstract: | A rapid, simple and reproducible single bounce attenuated total reflectance (SB-ATR) Fourier transform infrared (FTIR) spectroscopic method was developed for determination of the n − 6, n − 3 and ratio of n − 6:n − 3 polyunsaturated fatty acids (PUFAs) in poultry feed lipids using partial least squares (PLS) regression. Data for n − 6, n − 3 and ratio of n − 6:n − 3 was acquired by gas chromatography (GC) and used as a standard values for FTIR calibration. The best regression results were achieved using first derivatives of the 1475–650 cm−1 spectral region for n − 6, n − 3 and ratio of n − 6:n − 3 with high regression coefficients (R2) of 0.999, 0.994 and 0.998, respectively and low RMSEP values of 1, 0.06 and 0.83, respectively. The results of the present study revealed that FTIR could be used for rapid and accurate determination of n − 6, n − 3 and ratio of n − 6:n − 3 PUFAs present in poultry feed lipids. |
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| Keywords: | Abbreviations: FTIR, Fourier-transform infrared SB-ATR, single bounce-attenuated total reflectance PLS, partial least squares PUFA, polyunsaturated fatty acids GC, gas chromatography MS, mass spectrometry RMSEC, root mean square error of calibration RMSECV, root mean square error of cross validation RMSEP, root mean square error of prediction PRESS, predictive residual error sum of squares PRD, percentage relative difference SFA, saturated fatty acid MUFA, mono unsaturated fatty acid PF, poultry feed |
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