The auxiliary determination of the binding site of berberine binding to human serum albumin by surface-enhanced Raman scattering |
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Authors: | Wei ZhangYu Zhao Xueyuan BaiGe Hui John R. LombardiDaqing Zhao Bing Zhao |
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Affiliation: | a State Key Laboratory for Supramolecular Structure and Materials, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China b Center for New Drugs Research, Changchun University of Traditional Chinese Medicine, Changchun 130117, PR China c Department of Chemistry, The City College of New York, New York, NY 10031, United States |
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Abstract: | We report on the joint application of fluorescence, ultraviolet-visible (UV-Vis) and Raman spectroscopy to the study of berberine with human serum albumin (HSA). We propose the surface-enhanced Raman scattering (SERS) technique to improve the understanding of the quenching interaction caused by berberine which could be applied in recognition process of fluorescent drugs with large biomolecules. The fluorescence and UV-Vis spectroscopic results show that the fluorescence intensity of HSA is significantly decreased in the presence of berberine, and the quenching mechanism is static. The SERS technique demonstrates clear advantages over direct measurements in physiological conditions. By means of this method, we are able to deduce important information concerning the binding property of berberine when interacting with HSA. We show the nitrogen atom is free but the dioxolane is involved in the spontaneously electrostatic inducement and subsequently hydrophobic binding. |
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Keywords: | Raman Surface-enhanced Raman scattering (SERS) Binding site Berberine Human serum albumin (HSA) Orientation |
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