Template-directed synthesis in 3'- and 5'-direction with reversible termination |
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| Authors: | Andreas Kaiser Sebastian Spies Tanja Lommel Clemens Richert |
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| Institution: | 1. Institut für Organische Chemie, Universit?t Stuttgart, 70569 Stuttgart (Germany);2. Product Development, Research and Quality, Thyssen Krupp Rasselstein AG, 56626 Andernach (Germany) |
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| Abstract: | Extending both ways: A method for DNA-templated synthesis on solid support is described. Controlled, stepwise chain extension was demonstrated both in the direction favored by nature (3'-extension; see scheme) and in the direction typical for conventional DNA synthesizers (5'-extension). |
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| Keywords: | DNA solid‐phase synthesis primer extension protecting groups reversible terminator |
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