Determination of dextropropoxyphene and norpropoxyphene in plasma by high-performance liquid chromatography. |
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Authors: | K J Pettersson L B Nilsson |
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Institution: | Department of Bioanalysis, Astra Arcus AB, S?dert?lje, Sweden. |
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Abstract: | A simple and sensitive procedure for the routine assay of the analgesic drug dextropropoxyphene and its main metabolite, norpropoxyphene, in plasma is described. After liquid-liquid extraction from alkalinized plasma and back-extraction into a small volume of an acidic aqueous phase, the aqueous phase was injected into a column packed with 3-microns octadecylsilica particles. Ultraviolet absorbance detection at 210 nm was used. Concentrations down to 2 nM could be determined for both compounds; at this level, the intra-assay coefficient of variation was 5%. |
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