1,2-Diarylethylenediamines as pre-column fluorescence derivatization reagents in high-performance liquid chromatographic determination of catecholamines in urine and plasma |
| |
| Authors: | Y Umegae H Nohta M Lee Y Ohkura |
| |
| Institution: | Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan. |
| |
| Abstract: | Meso- and dl-1,2-diarylethylenediamines (14 species) were evaluated for pre-column fluorescence derivatization reagents in the high-performance liquid chromatographic determination of catecholamines (norepinephrine, epinephrine and dopamine) in human urine and plasma. Of the compounds, meso-1,2-bis(4-methoxyphenyl)ethylenediamine was most preferable for all the catecholamines in terms of sensitivity and selectivity. The detection limit for each catecholamine is approximately 0.5 fmol in a 50-microliters injection volume. |
| |
| Keywords: | |
|
|
