摄食转牙鲆生长因子基因集胞藻的大菱鲆血液指标及微核研究

以实验室养殖的幼小大菱鲆和养殖场养殖大菱鲆幼鱼为研究对象，研究了转牙鲆生长因子基因集胞藻作为饲料添加剂对鱼类血液生理生化指标和红细胞微核率及核异常率的影响。研究结果显示，转基因集胞藻对大菱鲆血液红细胞和白细胞数量、血红蛋白含量及红细胞体积没有影响（p〉0.05）；1.0％添加剂量没有引起血清转氨酶活性及碱性磷酸酶和乳酸脱氢酶活性增加（p〉0.05），实验组血清蛋白质、葡萄糖、甘油三脂、胆固醇、尿素氮和肌酐含量以及钾、钠、氯、磷浓度与对照组之间也不存在显著性差异（p〉0.05）；转基因集胞藻对红细胞微核及核异常没有诱导作用。结果表明转基因集胞藻不会引起大菱鲆组织器官损伤，也没有遗传毒性，是一种安全的转基因饲料添加剂，可以用作大菱鲆促生长剂使用。

Study on Haematology and Micronucleus of Turbot (Scophthalmus maximus L.) Fed with Transgenic Synechocystis sp.PCC6803 Containing Flounder GH Gene

The present study investigated effects of transgenic Synechocystis sp.PCC6803 containing flounder GH gene as feed additive on blood physiological and biochemical indices and peripheral blood erythrocyte micronucleus and other nuclear abnormality using juvenile turbot cultured in lab and in fish farm.Results showed that transgenic Synechocystis had no influence on blood cell count,haemoglobin content and mean corpuscular volume(p>0.05). 1.0% additive dosage of transgenic alga caused no increase in activities of aminotransferases,alkaline phosphatase and lactate dehydrogenase in serum(p>0.05).No significant differences(p>0.05) were observed between experimental and control group in total protein,glucose,triglyceride,cholesterol,urea nitrogen,creatinine levels,and sodium,potassium,chloride and phosphorus concentrations(p>0.05).Feeding transgenic Synechocystis did not induce the formation of micronucleus and other nuclear abnormalities in erythrocyte.Results of the present study indicate that transgenic Synechocystis can not impair organs of turbot and has no genotoxic effect on the fish,it is a safe transgenic feed additive and can be used as growth promoter for turbot.