Silica sol/Nano-Au/PVP修饰金电极电致化学发光测定槐定碱

基于槐定碱对联吡啶钌(Ru(bpy)2+3)在电致化学发光中的增敏机理,通过传感器修饰技术,将Silica sol/Nano-Au/PVP修饰到金电极表面,建立了简单、快捷测定槐定碱的新方法。实验表明,最佳实验条件下,在1.6×10-7~1.6×10-4mol/L范围内,槐定碱的浓度与其对应的电化学发光强度值呈良好的线性关系,I=4.000×107c+262.2,r2=0.994 5,检出限(S/N=3)为3.5×10-9mol/L。连续平行测定浓度为1.6×10-5mol/L的槐定碱溶液6次,其相对标准偏差(RSD)为2.9%。该方法具有较高的选择性和灵敏度,样品处理简单快速,用于槐定碱的测定,结果满意。

Determination of Sophoridine by Electrochemiluminescence Based on Nano-Au/Silica sol/PVP Modified Gold Electrode

In order to establish a simple and rapid method for the determination of sophocarpine,the gold electrode modified with silica sol/Nano-Au/PVP was prepared,which could be used to detect sophoridine by ECL based on the obvious electrochemiluminescence sensitization effect of sophoridine on Ru(bpy)2+3.Under the optimum conditions,the ECL intensities presented a good linearity with concentration of sophoridine in the range of 1.6×10-7-1.6×10-4 mol/L,with a linear equation of I=4.000×107c+262.2(r2=0.994 5) and a detection limit(S/N=3) of 3.5×10-9 mol/L.RSD(n=6) for determination of 1.6×10-5 mol/L sophoridine was 2.9%.The proposed method showed a high selectivity,sensitivity and simplicity,and could be applied in the analysis of sophoridine with satisfactory results.