Determination of mitomycin C in urinary bladder tissue by reversed-phase high-performance liquid chromatography

A procedure was developed for the determination of an anticancer drug of mitomycin C (MMC) in urinary bladder tissue samples by reversed-phase high-performance liquid chromatography with spectrophotometric (365 nm) and mass-spectrometric detection with detection limits of 50 and 10 ng/mL, respectively (signal-to-noise ratio = 3). The tissue samples were homogenized and solid-phase extraction was performed on a DSC-18 cartridge. The procedure was used for comparing the effectiveness of the MMC penetration into the urinary bladder tissue by passive diffusion and by intravesical drug electrophoresis. EDMA increases the rate of mitomycin penetration into urinary bladder walls by 3 to 5 times with respect to passive diffusion.