涡流色谱技术在生物样品分析中的应用

生物样品的复杂性使其在进行分析测定前必须经过处理。传统的样品前处理方法(如液-液萃取、固相萃取等)耗时长且操作繁琐。涡流色谱作为在线萃取技术,可以实现生物样品直接进样,减少了样品处理步骤,有效富集纯化了分析物,是一种高通量、高选择性的生物样品前处理方法。为此,本文介绍了涡流色谱技术的原理及优势,并总结了不同涡流柱的特点及其在生物样品分析领域中的应用情况。

Application of turbulent flow chromatography in the analysis of biological samples

The complexity of biological fluids makes the sample preparation necessary in the analytical process. The traditional techniques, such as liquid-liquid extraction and solid-phase extraction, are time-consuming and labor-intensive. Turbulent flow chromatography (TFC), as an on-line direct injection technique, possesses the advantages of reducing sample preparation steps and enabling the effective pre-concentration and clean-up of biological fluids. Therefore, it is a high-throughput and high-selectivity technique for biological sample pretreatment. In this review, the theory, advantages, characteristics and current status of TFC in bioanalysis are introduced and summarized.