EFFECT OF INTENSITY AND WAVELENGTH OF FLUORESCENT LIGHT ON CHROMOSOME DAMAGE IN CULTURED MOUSE CELLS |
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| Authors: | Ram Parshad Katherine K. Sanford William G. Taylor Robert E. Tarone Gary M. Jones Anne E. Baeck |
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| Affiliation: | Department of Pathology, College of Medicine. Howard University, Washington, DC, 20059 and Laboratory of Cellular and Molecular Biology and Biometry Branch, National Cancer Institute, Bethesda, MD 20014. U.S.A. |
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| Abstract: | Abstract—A single 3- to 20-hr exposure of line NCTC 9266 mouse cells to cool-white fluorescent light (4.6 W/m2) produces chromatid breaks and exchanges. The effective wavelength is in the visible range and coincides with the mercury emission peak at 405 nm. Increasing light intensity from 4.6 W to 15.3 W/m2 for 20 h causes a concomitant increase both in production of chromosome damage and formation of hydrogen peroxide (H2O2) in the serum-free medium. Cells washed free of medium and illuminated in saline for 3 h show chromosome damage to the same extent as cells illuminated in culture medium. Addition of catalase during the exposure period of 3 h eliminates the light-induced damage. We conclude that the light-induced chromatid breaks and exchanges result from H2O2 production within the cell and that exogenous catalase can enter the cell and prevent the damage. |
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