| DNA Separation by Capillary Electrophoresis with Ultraviolet Detection using Mixed Synthetic Polymers |
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| 作者姓名： | QianWANG XuXU |
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| 作者单位： | StateKeyLaboratoryofOrganometallicChemistry,ShanghaiInstituteofOrganicChemistry,ChineseAcademyofSciences,Shanghai200032 |
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| 基金项目： | 中国科学院知识创新工程项目 |
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| 摘 要： | The mixtures of two polymers, poly (N,N-dimethylacrylamide) (PDMA) and polyvinylpyrrolidone (PVP) were synthesized and used as the separation medium for double-stranded and single-stranded DNA fragments by capillary electrophoresis with UV detector. On optimal conditions, 2%w/v PDMA 2%w/v PVP can be used to separate the doublet 123/124bp in pBR322/Hae Ⅲ Markers.
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| 关 键 词： | DNA 分离 毛细管电泳分析 紫外线探测 合成聚合体 脱氧核糖核酸 |
DNA Separation by Capillary Electrophoresis with Ultraviolet Detection using Mixed Synthetic Polymers |
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| QianWANG XuXU.DNA Separation by Capillary Electrophoresis with Ultraviolet Detection using Mixed Synthetic Polymers[J].Chinese Chemical Letters,2003,14(12):1278-1280. |
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| Authors: | Qian WANG Xu XU |
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| Abstract: | The mixtures of two polymers, poly (N,N-dimethylacrylamide) (PDMA) and polyvinylpyrrolidone (PVP) were synthesized and used as the separation medium for double-stranded and single-stranded DNA fragments by capillary electrophoresis with UV detector. On optimal conditions, 2%w/v PDMA + 2%w/v PVP can be used to separate the doublet 123/124bp in pBR322/Hae III Markers. |
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| Keywords: | Synthetic polymer DNA separation capillary electrophoresis ultraviolet detection |
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